Toxicity testing of Greska’s Carbon-60™ for was conducted at the Knoebel Institute for Healthy Aging (KIHA),

The University of Denver, located in Denver, Colorado.

In his continued testing, Dr. Parades was interested in taking the toxicity experiment to the next level. Given the reported antioxidant properties of Carbon 60, Dr. Paredes investigated the potential of Greska’s Carbon-60 on cell protection upon confronting the cells with an extremely oxidant environment. In the next set of experiments, the goal was to observe Greska’s Carbon-60™ reaction when hydrogen peroxide (H2O2) was added to the cell culture media to induce cell death.

Hydrogen peroxide is a potent oxidizing agent and can be used for bleaching, as a disinfectant, and even as a rocket fuel component. In the scientific research community, it is common practice that hydrogen peroxide is used to induce cell death in scientific research and experiments.

Using the same rat brain cell culture as was used in the toxicity testing, the control sample and Greska’s Carbon-60 were prepared. After the addition of the hydrogen peroxide, following standard procedure, the expectation is to see significant cell death within 24 hours. When both samples were evaluated after 24 hours, the control sample (without Greska’s Carbon-60™) showed 80% cell death. What was astounding was the brain cell culture with Greska’s Carbon-60™ added, when checked at the 24-hour point, there was only 40% cell death. That is an increase in the survival rate of 50% when compared to the non-treated cells.

According to Dr. Parades, “Adding hydrogen peroxide to this cell culture is like throwing an atomic bomb in the petri dish. There’s nothing that can prevent cell death. To see this level of survival rate is remarkable.” Dr. Parades continues, “Even though this experiment was done in rodent cells, there is a great probability that such protective effect of the Greska’s Carbon-60™ that we observed, also happens in a whole organism, including humans, this result merits further research to fully explore the potential of this molecule (Greska’s Carbon-60) in the treatment of devastating conditions where oxidative stress is at the core of cell death”