Author: Tim Hughes

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Biome Study

Written by: Tim Hughes

Vagina Biome Study

The BiomeFx microbiome study was focused on 4 study candidates with varying imbalances in their reproductive organs, from pain and discomfort to irregular cycles. The ladies included in the study were of different nationalities and activity levels, from sedentary to an athlete, and included pre, peri, and post-menopausal. None of these women had ever used Carbon-60 prior to performing their baseline test. The testing included 15 days of nightly application of Greska’s C-60 both orally (2/3 ml) and intravaginally (1 ml).
Note: no other changes to diet or lifestyle were made during the test duration.

Lactobacillus is the predominating protective bacterial species in a woman’s vagina. When this is out of balance, this can lead to other more opportunistic species of bacteria and fungi populating the terrain. This can be seen clearly with the whole genome sequencing provided by the testing. 

What is truly profound
Greska’s Carbon-60;
showed anti-inflammatory benefits that appeared to have allowed the microbiome to create
beneficial shifts.

Before the application of C-60, the levels of the lactobacillus in some cases were so low their presence was “non-detectable.” Post-testing (after Carbon 60) demonstrated lactobacillus started to proliferate in the vagina.

Application:
15 days of nightly application of Greska’s C-60 both orally (2/3 ml) and intravaginally (1 ml). Note: no other changes to diet or lifestyle were made during the test duration.

Conclusion:
Symptom reductions were noted by each participant.
Utilizing Greska’s Carbon-60 for managing vaginal microbiome imbalances appears to be promising. Carbon 60 showed beneficial shifts in the vagina’s protective Lactobacillus bacteria.

Tests were conducted using: 
BiomeFx CosmosID 
Testing performed by:
WHOLEistic Lyfe
Practitioner:
Alyssa Blue, FDNP
Sarasota, Florida
813-461-4474
11/07/2022

 

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The Science of Carbon 60

Written by: Tim Hughes

A Better You at any Age!

“Using Greska’s C60, I can say for certain that my joint pain is finally gone. Better energy, better sleep, and better memory, and much more! I use just 1 dropper per day. Thank you, Bob!”           Greg V.

I have not been sick in two years since I’ve been taking Bob Greska’s Carbon 60. I do not have the aches and pains of a middle-aged woman. I sleep better. My overall health has improved.

           Kelli D.

Let’s look at the science behind Carbon 60

Greska’s Carbon-60 contains 60 carbon atoms—an ingredient commonly referred to as C60, Carbon 60, or fullerene. Scientists confirm that C60 exhibits a unique antioxidant property.  That’s exciting news since antioxidants help absorb free radicals. reduce oxidative stress—a significant part of chronic and degenerative illness.

Why take Greska’s Carbon-60

Health experts agree that oxidative stress and toxins contribute to many health issues, including age-related diseases.

Carbon-60 has been shown to protect you against oxidative stress and free radical buildup. That’s because Carbon 60 provides electrons to cells and toxins, resulting in reduced oxidative stress.

Studies have shown that Carbon 60 can provide anti-viral properties related to flu and other viruses. Research continues as investigators consider how C60 can impact other health conditions, including anti-aging skincare products.

When used topically for sore muscles and joints, Greska’s Carbon-60 quickly absorbs through the skin into the body. C60 can help suppress joint inflammation and supporting bone health, based on orthopedic research about fullerenes.

This is welcome news for those of you who seek an active lifestyle, whether at the gym or outdoors, creating a better you at any age.

Carbon-60 can also be part of a skincare routine; C60 showed a reduction in the appearance of wrinkles, according to a dermatology study.  Another study demonstrated an anti-inflammatory response in a group of people with acne sores.

Greska’s Carbon-60 was tested for toxicity by the University of Denver’s Knoebel Institute for Healthy Aging and showed no signs of toxicity in rodent cerebellar granule cells.  The University of Denver also found Greska’s Carbo-60 increased cell survival rates by 50% compared to untreated cells demonstrating the ability of Greska’s Carbon-60 to reduce oxidative stress.

What Makes Greska’s Carbon-60 Unique?

Greska exclusive Ultra Clean Carbon-60 powder conversion process produces a pure, bio-compatible, and bio-available form of Carbon 60. It is non-crystalline, nano-sized spheres are small enough to penetrate cells and mitochondria. Multiple third-party tests have verified Greska’s purity and lack of contaminants. These certification tests help customers know what’s inside their bottle of Greska’s Carbon-60 every time they buy.

Greska blends Carbon-60 into organic, non-GMO sunflower oil. And because of the known health benefits from quality sourced sunflower oil, it complements the benefits of Carbon 60.  Sunflower Oil contains vitamins E, A, C, and D, which are beneficial to the skin, can help regenerate new skin cells, reduce the appearance of wrinkles, and helps your skin rid itself of acne-causing bacteria.  The vitamin E and oleic acid in sunflower oil combined with the benefits of Carbon-60 can help protect the collagen and elastin in the skin and reduce the appearance of fine lines in your face, and prevent UV damage.

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Greska’s Safety Testing

Written by Sam Greska

SAFETY TESTS OF GRESKA’S CARBON-60™

Here are the results of safety testing conducted at the Knoebel Institute for Healthy Aging (KIHA), University of Denver and  Steep Hill Laboratories in Honolulu, Hawaii.

We know Greska’s Carbon-60™ was safe for human consumption, as we are 100% certain our proprietary non-solvent method of producing our C60 uses NO SOLVENTS at any step of the manufacturing process. We then add our C60 to high quality food grade organic sunflower oil. In order to provide the best quality product to our customers, our objective was to verify and provide documentation to our customers that Greska’s Carbon-60™ was not toxic. We wanted confirmation, that not only was Greska’s Carbon-60™ manufacturing process non-toxic but there were no other toxic substances in our product.

PART ONE FOR TESTING GRESKA’S CARBON-60™ FOR TOXICITY

Testing of Greska’s Carbon-60™ for toxicity was conducted at the Knoebel Institute for Healthy Aging (KIHA), University of Denver. Located at the base of the Rocky Mountains in Denver, Colorado, KIHA was founded in 2010 when DU received a generous gift from Betty Knoebel. Mrs. Knoebel was the widow of Ferdinand “Fritz” Knoebel, who was a pioneer in the food services industry.

KIHA’s mission is to create and implement solutions for aging issues through multidisciplinary research, education and outreach. Greska’s Carbon-60™ testing was led by Daniel Paredes, Ph.D., Assistant Research Professor whose specialty is aging neurodegenerative disorders such as Parkinson’s, Alzheimer’s and Huntington’s Diseases.

This study was conducted on rat cells, not human cells, therefore we can only confirm that Greska’s Carbon-60™ in an aqueous dispersion is non-toxic to rats brain cells.

Samples of neuronal cell culture of rodent (cerebellar granule cells) were exposed to different concentrations of Greska’s Carbon-60™ in an aqueous dispersion.  After 24 hours, there were no signs of any negative effects in the viability, morphology or cell death process as comparted to the non-treated cells.

The test results from KIHA of Greska’s Carbon-60™ being non-toxic was exciting news! Dr. Paredes continued testing and found other unexpected and interesting results.

In his continued testing Dr. Paredes was interested in taking the toxicity experiment to the next level. Given the reported antioxidant properties of Carbon 60, Dr. Paredes investigate the potential of Greska’s Carbon-60™ on cell protection upon confronting the cells with an extremely oxidant environment. In the next set of experiments, the goal was to observe Greska’s Carbon-60™ reaction when hydrogen peroxide (H2O2) was added to the cell culture media to induce cell death.

Hydrogen peroxide is a strong oxidizing agent and can be used for bleaching, as a disinfectant and even as a component in rocket fuel. In the scientific research community, it is common practice that hydrogen peroxide is used to induce cell death in scientific research and experiments.

In the research article, Is Hydrogen Peroxide a Suitable Apoptosis Inducer for All Cell Types?, it states,   “Hydrogen peroxide is currently the most widely used apoptosis inducer due to its broad cytotoxic efficacy against nearly all cell types.” 

Jinmei Xiang, Chunyun Wan, Rui Guo, and Dingzong Guo, “Is Hydrogen Peroxide a Suitable Apoptosis Inducer for All Cell Types?,” BioMed Research International, vol. 2016, Article ID 7343965, 6 pages, 2016. https://doi.org/10.1155/2016/7343965

HOW THE TESTS WERE CONDUCTED?

Using the same rat brain cell culture as was used in the toxicity testing, a control sample and Greska’s Carbon-60™ was prepared in an aqueous dispersion. After the addition of hydrogen peroxide, following standard procedure, the expectation is to see significant cell death within 24 hours. When both samples were evaluated after 24 hours, the control sample (non-Greska’s Carbon-60™) showed 80% of cells dead. What was astounding was the brain cell culture with Greska’s Carbon-60™ added, there was only 40% cell death reported. That is an increase in survival rate of 50% when compared to the non-treated cells.

According to Dr. Paredes, “Adding hydrogen peroxide to this cell culture is like throwing an atomic bomb in the petri dish. There’s nothing that can prevent the cell death. To see this level of survival rate is remarkable.”  Dr. Paredes continues, “Even though this experiment was done in rodent cells, there is a great probability that such protective effect of the Greska’s Carbon-60™ that we observed, also happens in a whole organism, including humans, these results merit further research to fully explore the potential of this molecule (Greska’s Carbon-60™) in the treatment of devastating conditions where oxidative stress is at the core of cell death”

SO WHY DID GRESKA’S CARBON-60™ SLOW THE CELL DEATH CAUSED BY THE HYDROGEN PEROXIDE?

Before we get to further explanation of the test results, let’s review these definitions so that we can better understand how free radicals, oxidative stress, reactive oxygen species (ROS) and antioxidants fit together.

Free radicals
  • Are naturally occurring due to metabolism (exercise)
  • May also be caused by UV radiation, tobacco smoke, environmental pollution, toxic chemicals, infections, or poor diet
  • Have a missing electron
  • Are unstable and highly reactive (constantly looking for an electron to repair itself, causing damage to the neighboring molecule causing a chain reaction or domino effect
  • Can damage cells, tissue and DNA through oxidation (oxidative stress)
Reactive Oxygen Species (ROS)
  • ROS are signaling molecules
  • Body’s natural defenses to detoxify or repair damage
  • Environmental stress (UV or heat) can cause ROS to increase
  • ROS increase can cause significant cell damage to lipids, proteins and DNA
  • Hydrogen peroxide is considered ROS
Hydrogen Peroxide
  • Kills cells by starting chemical reaction via lipid peroxidation
  • Free radicals “steal” electrons from the lipids in cell membranes
Oxidative Stress
  • Is an imbalance between free radicals and antioxidants
  • Occurs when there is an excess of free radicals
  • Cumulative cell damage caused by excessive ROS,
  • Oxidation is a chemical process that produces free radicals
Antioxidant
  • Molecules or compounds that inhibit oxidation by donating an electron to a free radical without becoming unstable themselves
  • Can be a food, naturally occurring, or dietary supplement
  • Support our immune system by attacking foreign bodies

So, we ask the question again—why did Greska’s Carbon-60™ slow down the cell death caused by the hydrogen peroxide?  A simple explanation is, it seems as if Greska’s Carbon-60™ is acting as an antioxidant. Let’s look at the chemical composition for hydrogen peroxide. Hydrogen peroxide is H2O2. That is, 2 atoms of hydrogen and 2 atoms of oxygen. If you remember from your basic chemistry lesson, water is H2O, which is 2 hydrogen atoms and 1 oxygen atom. Hydrogen peroxide is highly toxic to cells, causing cell death. It would appear that Greska’s Carbon-60™ was able to act as an antioxidant and neutralize one of the destructive oxygen atoms, thus dampening the toxicity of hydrogen peroxide. This is a significant and noteworthy finding that deserves further testing and research.

Now for the added bonus: Greska’s Carbon-60™ is so small that makes Greska’s C60 an incredibly effective antioxidant.

PART TWO OF TESTING GRESKA’S CARBON-60™ FOR SAFETY, WAS A TEST TO DETECT ANY TOXIC HEAVY METALS

As the second step in confirming Greska’s Carbon-60™ is non-toxic and safe, we tested for heavy metals at Steep Hill Laboratories.

Steep Hill Laboratories, with many locations throughout the United States is a highly prestigious science and technology company focusing on lab testing, licensing, genetics, and research and development for the cannabis industry.

Greska’s Carbon-60™ Heavy Metals Test Results

We are pleased to report that the Steep Hill Certificate of Analysis (COA) for Greska’s Carbon-60™ shows that our product contains none of these heavy metals:  Arsenic, Cadmium, Lead or Mercury.

WHAT DO: NOT DETECTED (ND), LIMIT OF DETECTION (LOD), AND BELOW LIMIT OF QUANTIFICATION (LOQ) REALLY MEAN?

Not Detected (ND) means the substance being tested for was not found

Limit of Detection (LOD) is a term used in analytical chemistry to define the lowest quantity of any substance that can be detected with any analytical procedure.

Limit of Quantification (LOQ) is the smallest concentration of a substance that can be reliably measured by an analytical procedure.

So to review, the Steep hills laboratory report shows that no heavy metals were found in Greska’s Carbon-60™.

In this installment of “The Truth Continues: Testing Greska’s Carbon-60™ for Safety” we will share the results of safety testing conducted at the Knoebel Institute for Healthy Aging (KIHA), University of Denver and  Steep Hill Laboratories in Honolulu, Hawaii.

There have been many scientific and research studies conducted regarding the toxicity of Carbon 60 and it has been determined that isolated C60 by itself is not toxic. In fact, the Baati rat study published in 2012 was initially intended as a toxicity test. What the scientists found was unexpected! Not only was C60 is not toxic to rats but it appeared to extend the lifespan of the rats that were given C60.

We know Greska’s Carbon-60™ was safe for human consumption, as we are 100% certain our proprietary non-solvent method of producing our C60 uses NO SOLVENTS at any step of the manufacturing process. We then add our C60 to high quality food grade organic sunflower oil. In order to provide the best quality product to our customers, our objective was to verify and provide documentation to our customers that Greska’s Carbon-60™ was not toxic. We wanted confirmation, that not only was Greska’s Carbon-60™ manufacturing process non-toxic but there were no other toxic substances in our product.

PART ONE FOR TESTING GRESKA’S CARBON-60™ FOR TOXICITY

Part One of testing of Greska’s Carbon-60™ for toxicity was conducted at the Knoebel Institute for Healthy Aging (KIHA), University of Denver. Located at the base of the Rocky Mountains in Denver, Colorado, KIHA was founded in 2010 when DU received a generous gift from Betty Knoebel. Mrs. Knoebel was the widow of Ferdinand “Fritz” Knoebel, who was a pioneer in the food services industry.

KIHA’s mission is to create and implement solutions for aging issues through multidisciplinary research, education and outreach. Greska’s Carbon-60™ testing was led by Daniel Paredes, Ph.D., Assistant Research Professor whose specialty is aging neurodegenerative disorders such as Parkinson’s, Alzheimer’s and Huntington’s Diseases.

This study was conducted on rat cells, not human cells, therefore we can only confirm that Greska’s Carbon-60™ is non-toxic to rats brain cells.

Samples of neuronal cell culture of rodent (cerebellar granule cells) were exposed to different concentrations of Greska’s Carbon-60™.  After 24 hours, there were no signs of any negative effects in the viability, morphology or cell death process as comparted to the non-treated cell.

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What Is Carbon 60?

Written by: Sam Greska

Greska’s Carbon-60 is an antioxidant, and so much more. But before we get into the details, let’s go over some basic processes that contribute to aging in the body. What is an Antioxidant? An Antioxidant is a molecule (a group of atoms bonded together) That is so stable it has the ability to donate an electron to a free radical without losing its own stability. Now what is a free radical, and why does it need to be stabilized? What even is an electron? Let’s start with free radicals. Free Radicals are highly reactive and unstable molecules. These molecules (free radicals) are unstable because they are missing an electron thus becoming unpaired. Usually, these subatomic particles (electrons) are orbiting atoms but due to movement, or toxins they get damaged and the little electron goes flying off or gets stolen by another molecule. Thus making you guess it, a free radical.

These free radicals are missing an electron due to damage caused by running, working out, injuries, toxins, and much more. Really anything your body does produces free radicals, even the process of your body making food into energy creates free radicals.

But as we age our body loses its ability to fight free radicals efficiently enough to fix the problem and get rid of oxidative stress. We take longer to heal and our body has to allocate more energy to just maintain itself. This is why we lose energy as we age. Most diseases are also caused by an overabundance of free radicals.

This simple yet infinitely complex process can produce a domino effect, causing oxidative stress. Oxidative stress is caused when your body produces more free radicals than your body has the ability to fight in a given area. Many times your body just needs time to catch up. The day after a workout when your muscles are still sore is mainly caused by oxidative stress. After a few days, your body regains control and wins the battle against these free radicals.

So what is Carbon 60 and how can it help? Most antioxidants we get are found in foods, but these are large and bulky they often don’t have the ability to get around the body and reach everything.

But Carbon 60 is an antioxidant that is made up of only 60 atoms, thus the name Carbon 60! This molecule can travel throughout your body with ease reaching down to the subcellular level. Why does size help? Well, it’s easier to move around the body. It has the ability to find problematic molecules (free radicals) faster than any antioxidant known to man! It is truly a great discovery that will help and, is helping many people around the world!

What Makes Greska’s Carbon-60 Unique?

Greska’s Carbon-60 is unique due to the fact that it is the only food-grade Carbon 60 on the market. Carbon 60 is difficult to produce and the main method is to use solvent, such as toluene (paint thinner) to extract the Carbon 60 from soot that is made with Carbon rods and high voltage. Bob Greska invented a new method to produce Carbon 60 using no solvents, resulting in a pure, clean Carbon 60 that is more bioavailable due to the small nano size. Greska’s Carbon 60 is the only solvent-free Carbon 60 on the market today.

Greska’s Carbon-60 has over 100 billion molecules of Carbon 60 per serving, making it a readily available source for antioxidants.

Greska’s Carbon-60 is also one of the smallest antioxidants known to date. Carbon 60 gets through to the smallest places in the body.

Why We Believe Greska’s Carbon-60 Is the Best Product On the Market?

Greska’s Carbon-60 is one of the most cost-effective brands on the market. Most all other known brands are at least 2-4 times more expensive per serving.

Greska’s Carbon-60 is the most potent product on the market, Greska’s Carbon-60 is black in oil whereas other companies do not have the ability to saturate their oil past a light amber color, due to solvent laden Carbon 60.

Greska’s Carbon-60 is the cleanest product on the market. With no use of solvent anywhere Greska is the only company that is food-grade.

Greska’s Carbon-60 is the most bioavailable Carbon 60 on the market. Being made up of naturally formed nanospheres, Greska’s Carbon-60 has smaller molecule clusters than any competitor.

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What is Bioavailability and why is it important?

Written by: Sam Greska

What is Bioavailability and why is it important? Before we get too technical, it’s important to have a basic understanding of bioavailability and antioxidants.

Carbon 60: is a soccer ball-shaped molecule composed of 60 Carbon atoms. Known as a free radical scavenger, C60 has the ability to share/give electrons away to reduce oxidative stress. Because C60 is so small, it has the ability to penetrate muscles, bones, organs, and into cells and the mitochondria. Studies have indicated Carbon 60 has anti-bacterial, anti-viral, and anti-fungal properties and reduces inflammation to support healthy joints.

Antioxidant Definition- An antioxidant protects the cells in your body against free radicals by readily donating an electron to the free radical, thereby stabilizing the free radical.

Free-Radical: Free radicals and toxins are molecules in your body missing an electron. These molecules then steal electrons from healthy molecules. Which, in turn, steals an electron from its neighbor. This domino-like effect of stealing electrons is known as Oxidative Stress and is very damaging to cell function. Greska’s Carbon 60 provides electrons to molecules and toxins, resulting in a reduction in oxidative stress. 

Bioavailability Definition- The level and rate that a substance (such as an antioxidant) is distributed into the body or is made available. Something that is highly bioavailable has a higher ability to freely maneuver around the body. The smaller the molecule, the more bioavailable it is.

Greska’s Carbon-60 is a highly Bioavailable antioxidant. The most important factors that make it so bioavailable are the size, purity and spherical geometry of the C60 molecule. It is about 4000 times smaller than antioxidants found in red wines, berries and dark greens. Since Greska’s C60 molecules are so small, their potency and bioavailability are dramatically increased.

Each Carbon 60 Molecule has the ability to share, or donate, electrons to stabilize free radicals and neutralize toxins: a super antioxidant. Completing, by supplying the missing electron, turn these molecules back on giving your body more energy to mend itself naturally.

We are the only “Food Grade” Carbon 60 manufacturer in the world.

We use a solvent-free process, (as we use no toxic solvents anywhere in our process).

We produce only Non-Solvent Extracted (Non-SE) Carbon 60.

Our sole and exclusive manufacturing process produces pristine, pure raw Carbon 60. Greska’s proprietary process produces pure C60 spheres approximately 4000 times smaller than ALL other solvent extracted methods used by our competitors. Our scientific advancement of manufacturing Non-SE Carbon 60 was developed in 2012 by a 40-year experienced Aerospace Materials and Processes Carbon Engineer.

We use a highly filtered non-GMO, Certified Organic Sunflower as carrier for the Carbon 60.

Our many customers proclaim that Greska’s Carbon-60 delivers unsurpassed results for Energy, Health and Vitality. Many long term supplement consumers tell us that Greska’s Carbon-60 is the best nutritional supplement they have ever taken.